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Experimental model and categorization of effects of ancestral vinclozolin vs control lineage and CRS in rats. A, Experimental flowchart. Rats were exposed to vinclozolin or vehicle (dimethylsulfoxide) control 3 generations previously, and the F3 generation used for CRS during adolescence or no stress. All animals were then behaviorally tested as adults. B, Experimental groups and comparisons. Planned comparisons are shown, with comparisons 1, 3 and 5 the focal group of the study.
