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. 2014 Jul 25;155(10):3956–3969. doi: 10.1210/en.2013-2095

Figure 5.

Figure 5.

Effect of ghrelin (10nM) on leptin-stimulated neuronal firing. A, Representative neuronal recording of rat NG neuron with no treatment after electroporation of control siRNA. B, Representative neuronal recording of a NG neuron stimulated with leptin (10nM, Lep), followed by recovery of the membrane potential. C, representative neuronal recording of rat NG neurons stimulated with leptin (10nM) plus ghrelin (10nM, Ghr), D, Representative neuronal recording of NG neuron stimulated with leptin (10nM), 48 h after transfection with SOCS3 siRNA; and E, representative neuronal recording of NG neuron stimulated with leptin (10nM) plus ghrelin (10nM), 48 h after transfection with SOCS3 siRNA. F, Histogram shows that leptin (10nM) generated an inward current of 45.1 ± 8 pA in 30% of neurons tested. Ghrelin (10nM) significantly inhibited leptin-stimulated neuronal firing, reducing the inward current to 14.1 ± 5 pA. Transfection of NG neurons with SOC3 siRNA partially reversed ghrelin's inhibitory effect on neuronal firing. G, SOCS3 CY3-siRNA transfected/labeled neuron used for patch-clamp studies. The data are representative of six independent experiments. *, P < .05, significantly different from leptin-stimulated neuronal firing. **, Significantly different from leptin-plus-ghrelin–stimulated neuronal firing.