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. 2014 Jul 25;155(10):3956–3969. doi: 10.1210/en.2013-2095

Figure 6.

Figure 6.

Silencing of SOCS3 prevents the inhibitory action of ghrelin on leptin-stimulated nodose neuronal activity in vivo. A, Response of NG neuron electroporated with control siRNA to the administration of leptin (225 μg/kg, iv). B, After injection of ghrelin (25 μg/kg, iv), the same neuron failed to respond to leptin. C and D, Silencing the expression of SOCS3 did not affect leptin (225 μg/kg) -stimulated neuronal firing, but it abolished the inhibitory action of ghrelin (25 μg/kg) on leptin stimulation. E, Histogram summarizes NG response to leptin (225 μg/kg) before and after treatment with ghrelin in rats with NG electroporated with either control siRNA or SOCS3 siRNA. F, Success in knocking down SOCS3 gene expression in the NG was validated by RT-PCR analysis. GAPDH was used as the loading control. The data are representative of six independent experiments. *, P < .05, significantly different from basal. **, P < .05, significantly different from leptin-stimulated neuronal firing.