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. 2004 Jun;78(12):6636–6648. doi: 10.1128/JVI.78.12.6636-6648.2004

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FIG. 2. — PPPY defect cannot be complemented by overexpression or stabilization of Gag precursor. (A) ELISA determination of the percentages of total MA reactive protein present in the supernatants of 293T cultures transfected with pCMVHT1 (bar 1), pCMVHT1-P3G (bar 2), pCMVHT1-A3P (bar 3), pCMVHT1-P3A/A3P (bar 4), pCMVHT1-PTAPVEP3Y (bar 5), and pCMVHT1-PTAPVEP3G (bar 6). The average and standard deviations of five independent experiments are shown. (B) Relative total amounts of MA in each culture expressed as percentages of MA expressed by the pCMVHT-1 construct. The results of three experiments are summarized. Sample designations are the same as for panel A. (C) Percentages of total MA in the supernatants of 293T cell cultures transfected with pCMVHT1-PR⁻ (bar 1), pCMVHT1-PR⁻-P3G (bar 2), pCMVHT1-PR⁻-A3P (bar 3), and pCMVHT1-PR⁻-P3A/A3P (bar 4). (D) Percentages of total MA in the supernatants of 293T cell cultures transfected with pCMVHT1-NCYΔX (bar 1), pCMVHT1-NCYΔX-P3G (bar 2), pCMVHT1-NCYΔX-A3P (bar 3), and pCMVHT1-NCYΔX-P3A/A3P (bar 4).