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Late domain mutations do not affect the infectivity of the virus in single-cycle assays. wt and mutant pCMVHT-1-bearing viruses were cotransfected with pHTC-GFPluc and pCMV-VSVg into 293T cells. After 2 days, filtered supernatants were transferred to B5 cells. The luciferase activity was assayed at 2 days postinfection. The infectivity of the mutants is expressed relative to that of pCMVHT1, which was set to 100%.
