FIG. 6.

HTLV-1 late motif interacts with WW domains of WWP1. (A) Schematic representation of major domains of WWP1 (top) and TSG101 (bottom). The regions inserted into the activation domain fusion plasmids of the mammalian and yeast two-hybrid systems (pVP16 and pAS-2, respectively) are indicated by the bars. (B) pM-p19+ and its late domain mutant versions expresses the Gal4 DNA-binding domain fused to residues 5 to 154 of Gag. The reporter plasmid pGL5-Luc expresses the luciferase gene under the control of five copies of the Gal4 consensus binding site. Representative results from one of three experiments are shown. Activation is expressed as the fold induction over that seen with the pM-p19+/pVP16 combination.