TABLE 1.
Yeast two-hybrid interaction between HTLV Gag proteins and TSG101
| DNA-binding domain plasmid | Interaction with activation domain plasmida
|
||
|---|---|---|---|
| No insert | TSG101-UEV | WW domains (WWP1) | |
| HIV-Gag | − | ++ | − |
| HIV-p6 | − | + | − |
| HTLV-Gag | − | ++++ | ± |
| HTLV-MA | − | ++++ | ± |
| HTLV-MA-P3G | − | +++ | ND |
| HTLV-MA-A3P | − | − | ND |
| HTLV-MA-P3AA3P | − | − | ND |
a
+, interaction is present, as evidenced by the growth of blue colonies in the presence of X-Gal on medium without leucine, tryptophan, histidine, and adenine. The number of plus symbols indicates the level of activity in the β-galactosidase assay of replicated colonies. −, no growth and no interaction; ND, not done.