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. 2014 Sep 15;206(6):763–777. doi: 10.1083/jcb.201403108

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© 2014 Shen et al.

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Figure 2. — Vps32 polymerizes to form curved, single-stranded filaments. (A) A representative cryo-EM image (inverted) of Vps32. Select spiral pairs (purple), hybrid spirals (purple/green), and ring structures (blue) are highlighted. Bar, 50 nm. (B) A straight filament (yellow boxed region in A) was chosen for FFT analysis and width measurement (intensity after background subtraction was used to calculate the width of filaments). Layer lines in the FFT analysis are highlighted with arrows. Bar, 5 nm. The data shown are from a single representative experiment, which was repeated six times (n = 15 for the experiment shown). (C) Distribution of spiral diameters. Both inner and outer diameters of spiral filaments were measured in two axes (x and y). Data shown are from multiple experiments, repeated at least six times independently. AU, arbitrary unit.