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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Protein Expr Purif. 2014 Aug 11;102:76–84. doi: 10.1016/j.pep.2014.07.002

Fig 3.

Fig 3

Purification of hIL-12 using heparin Sepharose chromatography. (A) Fractionation of proteins, secreted in the hollow fiber culture medium, on heparin Sepharose. (B ) SDS-PAGE of the different fractions, eluted under a NaCl gradient, as detected by Coomassie blue staining. Lane 1 - Cell Culture Supernatant; Lane 2 - Flow through (100 mM NaCl); Lane 3 – 200mM NaCl; Lane 4 – 300mM NaCl; Lane 5 – 500mM NaCl; PM-Protein Marker. (C) Lane showing the purified hIL-12 from 500mM NaCl fraction stained with ProQ emerald green stain and a distinct p35 subunit band(s) is highlighted by an arrow.