FIG. 3.

Production of IFN-β by endothelial cells after infection with HTNV or TULV. (A) An ELISA technique was used to analyze supernatants from infected (MOI = 1) HUVECs collected at the time points indicated. As a positive control, supernatants from cells treated with poly(I · C) (10 μg/ml for 24 h) were included. Concentrations (in picograms per milliliter) are given as means ± standard errors of the means from three individual experiments with cells derived from three different donors. (B) Quantitative real-time RT-PCR analysis was performed to determine the relative copy numbers of IFN-β transcripts in infected (MOI = 1) HUVECs. As a control, cells were infected (MOI = 1) with UV-inactivated HTNV (mock infection). Results are means ± standard errors of the means from three individual experiments with cells from three different donors.