FIG. 7.

(A) Schematic diagram of the SV-1-env construct. Start codons for the expression of gag, tat, and rev were mutated. SV-1-env 3U contains a mutation in position +3 of 5′ ss no. 4. The positions of the primers used for RT-PCR are indicated by arrows. (B) Determination of the linear amplification range for SV-1-env and hGH RT-PCR assays. (C) RT-PCR assay. HeLa-T4⁺ cells were transfected with SV-1-env (lanes 1 and 2), SV-1-env SF2⁻ SRp40⁻ (lanes 3 and 4), or SV-1-env 3U (lanes 5 and 6) and cotransfected with pXGH5 expressing the hGH mRNA as an internal control for transfection efficiency. WT, wild type; +, present, −, absent.