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Consensus sequences for each HIV-1 subtype were aligned (20). The GAR ESE, the E42 fragment, and the predicted SF2/ASF and SRp40 binding sites are indicated. The 3′ and 5′ ss and Rev ATG translation initiation site are indicated. Since the GAR and the E42 fragment are both within the rev coding region, the extended sequence conservation within the GAR fragment indicates an added selective pressure to maintain the ESE sequence unchanged among different isolates.
