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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Methods Mol Biol. 2009;568:261–279. doi: 10.1007/978-1-59745-280-9_17

Fig. 6.

Fig. 6

Functional and phenotypic characterization of “side population” cells. Freshly isolated lung cancer cells (CD45- viable singlets) were divided into Ho33342 excluding (F) and retaining (G) populations. Rhodamine 123 (R123) exclusion and the surface expression of EpCAM, CD90 (Thy1) and light scatter properties (FSC, SSC) were evaluated. Side population cells (top panels) cotransported R123 (15.4%). Cells which cotransported Ho33342 and R123 were mostly EpCAM negative/CD90- and had low light scatter comparable to resting lymphocytes. Nonside population cells, which did not transport R123, were of mixed surface phenotype (EpCAM/CD90) and were predominantly (97.2%) large cells with high light scatter.