FIG. 1.

Structures of SIN HIV-based vectors and sequences of ICAM-2 promoters. (A) Schematic of SIN vectors. At top is the base vector, with U3 sequence and gene products as indicated. The bottom three are SIN vectors used in these studies; provirus deletions are indicated by the delimited bars. CMV and 340 denote the CMV IE enhancer and the ∼340-bp ICAM-2 promoters, respectively; X denotes the unique XbaI site just 5′ of the minimal 140 ICAM-2 promoter (140). (B) DNA sequence of ICAM-2 340 promoter. Sequence of ∼340-bp ICAM-2 promoter, beginning at −292 and ending at +44 (relative to major transcription start site), with elements bolded and identified in italic. (C) Minimal 140-bp ICAM-2 promoter, beginning at −95 and ending at +44; note absence of TATA box. DNA elements are identified, as are transcription start sites (bent arrows).