Analysis of four distinct subpopulations sorted from human stromal vascular fraction. Freshly isolated stromal vascular fraction from abdominal adipose tissue was stained for flow cytometric analysis using a combination of CD31, CD34, CD45, and CD146. (Above) Multiparameter flow cytometric sort strategy of human stromal vascular fraction showing four distinct subpopulations: endothelial mature (EM), endothelial progenitor (EP), pericyte (PERI), and preadipocyte/supra-adventitial adipose-derived stem cell (PA) populations. (Below) Morphologic images of stromal vascular fraction sorted subpopulations (original magniflcation, × 200). Scale bar = 10 μm for all images.