CD34 affinity agarose beads prepared with N,N′-Disuccinimidyl carbonate (DSC) reveal specific depletion of CD34 cells from ovine blood specimens. Blood specimens were incubated with no beads, commercial N-hydroxysuccinimide (NHS) sepharose beads conjugated to CD34 antibody, plain agarose beads, isotype control antibody (MOPC) DSC beads, or CD34 conjugated DSC beads (n = 3 for all conditions; standard error bars are shown). CD34-affinity DSC beads deplete CD34-positive cells in a statistically significant manner relative to isotype control affinity beads (P = .01).