Figure 6.

Miz-1 regulates EpoR signaling through Stat5 phosphorylation. A. Stat5/pStat5 Western Blot on lysates from Ter119⁺ splenocytes from WT and Miz-1-deficient mice stimulated (+Epo) or not (-Epo) with Epo. Actin and Ponceau staining are presented as loading controls. Blots are representative of at least two independent experiments. B. Ter119⁺ splenocytes were isolated from adult Miz-1^fl/fl vav-Cre and WT animals stimulated (+Epo) or not (-Epo) with Epo. RNA from these cells was reverse transcribed and cDNA was used for quantitative RT-PCR (QRT-PCR). Expression of indicated genes was normalized to the expression of Gapdh. Data represent two independent experiments each done in triplicates. Error bars indicate standard deviation. C. Miz-1 and IgG control ChIP in Ter119⁺ splenocytes. Data are presented as fold enrichment at indicated regions relative to beta-globin promoter and are representative of three independent experiments. Vamp4 promoter is the positive control [33] and Chr2 (intergenic region in chromosome 2) is the negative control.