FIG. 8.

(A). Schematic representation of various constructs of GGNNV protein A deletion mutants and N-terminal amino acids fused with GFP and subcellular localization of their derivatives of protein A in transfected Cos-7 cells. (B) Top panel, membrane association assays of protein A deletion mutants and N-terminal amino acids in transfected Cos-7 cells. Membrane fractions of Cos-7 cells transfected with protein A deletion mutants fused with GFP or N-terminal amino acids fused with GFP were extracted at 36 h posttransfection, and samples were separated by SDS-PGE, transferred to a membrane, and immunoblotted with anti-GFP sera. Bottom panel, equilibrium density Nycondenz gradient fractionation of lysates from EGFP-215-255-transfected Cos-7 cells. Equal-volume fractions were recovered from the top of the gradient, and samples were separated by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and immunoblotted with rabbit anti-GFP. The position of protein A is indicated on the right. The 50-kDa rabbit immunoglobulin G (IgG) heavy chain was detected by the secondary reagent, and its position is also indicated on the right.