FIG.2.

Maturation of DCs cultured from a chronically HCV-infected chimpanzee is achieved by both MCM mimic and CD40LT. (A) MCM mimic (TNF-α, IL-1β, IL-6, and PGE₂) or CD40LT (1 μg/ml) was added in a medium supplemented with 10% plasma from a noninfected chimpanzee to immature DCs from a chronically infected chimpanzee (CB0507) on day 5 of culture. On day 7, the DCs were harvested and labeled with monoclonal antibodies as indicated. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). (B) MCM mimic was added to immature DCs from an uninfected (A255B) or chronically infected (CB0507) chimpanzee on day 5 of culture. On day 7, the DCs were harvested and labeled with the indicated monoclonal antibodies. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). For both panels, the gates were set to include cells with large amounts of FSC and SSC. The data shown are for one representative experiment out of four.