Figure 3.

Primed CD8⁺CD25⁺ T cells expressed IL-10. Splenic cells were isolated on day 6 from IL-10-GFPtg mice infected with H5N1 virus and surface stained for CD8 and CD25. CD8⁺ T cells were separated into CD25 positive and negative cells. These cells were further divided into GFP positive or negative, with GFP serving as a marker for IL-10 positivity. The percentages of CD8⁺CD25⁺ T cells and CD8⁺CD25⁻ T cells that were IL-10⁺ or IL-10⁻ are shown together with results of statistical analysis. The dot plots represent one of five independent experiments with similar results (n = 5 mice).