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Overexpression of SOCS3 suppresses IFN-α-induced Tyr-phosohorylation of STAT1. FL cells were transiently transfected with the SOCS3 expression plasmid (pSOCS3). (A) The expression of SOCS3 was determined by Western blotting. (B) After 24 h of transfection, the cells were treated with IFN-α (1,000 IU/ml) for 10 or 20 min. The cell lysates were then analyzed for the Tyr phosphorylation status of STAT1 by Western blotting. Actin and STAT1 were used as controls. *, nonspecific binding.
