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. 2013 Oct 9;24(2):189–203. doi: 10.1002/hipo.22214

FIGURE 1.

FIGURE 1

GAD67 expression in GAD67-eGFP labeled cells of the dentate gyrus. (A) Z-projection of a confocal image stack of a horizontal slice (300 µm thickness, steps of 3 µm) derived from a P20 GAD67-eGFP knock-in mouse. Green spots indicate endogenous eGFP epiflorescence (excited at λ = 488 nm; emission band-pass at 500–550 nm) of interneuron cell bodies. (B) Densitometric measure of labeling intensity for GAD67 antibody labeling in single cell bodies plotted as a function of eGFP signal intensity. (C,D) Confocal image of eGFP-fluorescence. In green [C], endogenous eGFP; in red [D], immunolabeling against GAD67. Arrowheads point to eGFP- and GAD67- positive interneuron somata. (E–G) GAD67-eGFP co-localizes with interneuron-specific markers. Representative confocal images obtained from the dentate gyrus gcl-hilus border confirming co-localization of GAD67-eGFP with the common interneuron markers parvalbumin (PV; E), calbindin (CB; F) and calretinin (CR; G). Secondary antibody conjugated to Cy3 was directed against antibodies targeting PV, CB and CR. Bar graphs summarize the percental fraction of the neurochemically defined interneuron types. Abbreviations: gcl, granule cell layer; ml, molecular layer.