TABLE 1.
NoV RNA1 mutants
| NoV1 | Phenotype | Genotypeb | Protein sequence corresponding toc:
|
|
|---|---|---|---|---|
| B2 ORFd | A/B1 ORFe | |||
| WTa | B2++ | WT | MTNMS | MRLR |
| Mutant 1a | B2−− | U2745C, U2754C, C2757G | T..T* | .... |
| Mutant 2a | B2+− | U2754C | M..T. | .... |
| Mutant 6 | B2−+ | U2745C | T..M. | .... |
| Mutant 7 | RNA3− | C2731A, G2732A, U2733A, G2734A | No RNA3 | R904K, V905I |
a
Construction of the WT NoV1 cDNA and mutants 1 and 2 has been previously described (23).
b
Nucleotide numbers refer to positions in NoV RNA1.
c
A period indicates identity to WT.
d
Protein B2 is in the +1 reading frame with respect to the overlapping protein A ORF. The first five positions are indicated. The asterisk indicates a stop codon.
e
Residue 1 of protein B1 corresponds to residue 908 of protein A; protein B1 is identical to the C terminus of protein A. The first four positions are indicated.