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Processing of HCV core-E1 polyprotein in the human hepatoma cell line FLC-4. Flag-Core-E1-HA (lane 2), Flag-Core ASC/VLV-E1-HA (lane 3), Flag-Core LVL/3A-E1-HA (lane 4), or Flag-Core IF176/177AL-E1-HA (lane 5) was expressed in FLC-4 cells and analyzed by immunoblotting with anti-Flag (upper panel) or anti-HA (lower panel) antibody. Cells transfected with an empty plasmid were used as a negative control (lane 1).
