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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Circ Res. 2012 Jul 25;111(8):991–1001. doi: 10.1161/CIRCRESAHA.112.268110

Figure 2. RTEF-1-/- transgenic mice have lower IGFBP-1 levels and increased HOMA-IR.

Figure 2

A. Blood glucose levels were obtained from food-deprived (overnight), restrained, unanesthetized mice. Blood was obtained via submandibular bleed, and glucose was quantified using a CVS TRUEtrack glucose monitor at 8 weeks of age on WT and RTEF-1-/- mice fed a normal chow or high fat diet. Blood glucose was significantly higher in the transgenic mice that lacked RTEF-1 in the endothelium in both the NCD and HFD (*p=0.022; n = 18 mice). B. Serum insulin concentration was measured by ELISA with a maxiumum absorbance of 450 nm. The limit of sensitivity is at or above 0.121ng/ml. C. Serum IGFBP-I concentration was measured by ELISA. IGFBP-1 concentration was measured with a maximum absorbance of 450 nm. The limit of sensitivity is at or above 31.2 pg/ml. (n = 3; * p=0.035). The IGFBP-1 levels inversely correlated with blood glucose levels in these mice.