Table 1. HLA-A2.1 expression levels on spleen cells and ear skin tissues by flow cytometry and immunohistochemistry respectively.
| Group (Vaccine) |
Rabbit ID | HLA-A 2.1 | |
|---|---|---|---|
| Positive cells on spleen cells (M1%) | Positivity on ear tissue * | ||
| Group 1 (TT-E1peptide) |
3206 | 56.40 | ++/+++ |
| 3219 | 30.02 | +/++ | |
| 2914 | 38.49 | ++ | |
| 3214 | 37.52 | ++ | |
| Group 2 (E1 peptide) |
3207 | 32.51 | ++/+++ |
| 3222 | 56.70 | +++ | |
| 2916 | 28.15 | ++ | |
| 3216 | 31.82 | ++ | |
| Group 3 (E1 peptide-TT) |
3210 | 54.19 | ++ |
| 3223 | 57.14 | +/++ | |
| 2917 | 52.28 | ++/+++ | |
| 3217 | 36.48 | +/++ | |
| Group 4 (TT-control) |
3211 | 46.06 | +/++ |
| 3224 | 73.40 | ++ | |
| 2918 | 52.70 | +/++ | |
| 3218 | 44.91 | +/++ | |
*
grading of A2 positivity by microscope based on visual inspection. “+” to “ +++” represents from weak to strong A2 staining (as shown in supplemental Figure 1).