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. 2014 Sep 16;8:311. doi: 10.3389/fnbeh.2014.00311

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Copyright © 2014 Rahman, Kerskens, Chattarji and O'Mara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The experimental design. (A) A schematic representation of the experimental setup. (B) A diagrammatic representation of the experimental plan. The animals were handled for 2 days, and then the stressed rats were subjected to 2 h Immobilization Stress daily for a period of 10 days. On day 11, the rats were subjected to CO₂ inhalation test along with Magnetic Resonance Imaging (MRI). A high-resolution structural scan was carried out first. Then a continuous arterial spin labeling (CASL) scan was carried out. During the scan for CASL the amount of CO₂ in inhaled air was varied stepwise as indicated in the diagram.