Figure 5.

Characterization of mutant mice at P30 and P60. Analysis of ERG (A) and immunofluorescence analysis (B) were performed as described in the Materials and Methods section. Rhodopsin (red) and M-opsin (green) antibodies were used for staining at P30 (B). Nuclei were stained with Hoechst (blue). (C) Cell death at P18 and P30 was assessed by TUNEL staining (green) of retinas of indicated genotypes. Graphical representation from three different experiments of number of TUNEL-positive (green) cells in the ONL is depicted on the right. Nuclei are stained with Hoechst (blue). Scale bars: 20 μm. Number of mice in (A) is indicated as n. *P < 0.001.