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. 2014 Jul 25;15(7):404. doi: 10.1186/s13059-014-0404-4

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© Kakihara et al.; licensee BioMed Central 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of R2TP complex on Nop58 stability. (A) Western blot analysis of steady-state protein levels of box C/D snoRNP protein components in WT, rvb1-DAmP, rvb2-DAmP, pih1Δ, and tah1Δ strains in log phase cells. (B) Western blot analysis of protein levels of box C/D snoRNP core proteins in NOP56-FLAG pih1Δ and SNU13-FLAG pih1Δ strains transformed with p416GAL-PIH1 plasmid. Cells were shifted from galactose- to glucose-containing medium, and protein levels were analyzed as a function of time. (C) Western blot analysis of Nop58 and Pih1 steady-state levels in log phase WT and nop58-3′Δ cells.