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. 2014 Jul 25;15(7):404. doi: 10.1186/s13059-014-0404-4

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© Kakihara et al.; licensee BioMed Central 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analysis of R2TP complex interaction with mature and premature box C/D snoRNA. (A) Northern blot analysis of mature U14 snoRNA in pulldown complexes of Pih1-FLAG (R2TP) and Nop56-FLAG (box C/D snoRNP); 6 μg and 0.6 μg of total RNA and RNAs purified from the pulldown complexes are shown. WT (no tag) strain was used as a negative control. (B) RT-PCR analysis of premature U14 snoRNA in pulldown complexes of R2TP (Rvb1-, Rvb2-, and Pih1-FLAG) and box C/D snoRNP (Nop1-, Snu13-, Nop56-, and Nop58-FLAG). RNAs were purified from the pulldown complexes and PCR was performed with (+RT) or without (−RT) reverse transcription.