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. 2014 Jul 25;15(7):404. doi: 10.1186/s13059-014-0404-4

Figure 7.

Figure 7

R2TP nuclear import and export is dependent on Kap121 and Crm1. (A) Subcellular localizations of endogenously GFP-tagged Pih1 and Tah1 in wildtype background and in kap121-34 mutant cells at the permissive (26°C) and restrictive (30°C) temperatures in log phase cells. (B) Subcellular localizations of endogenously GFP-tagged Pih1 and Tah1 in WT cells expressing Nup53-CΔ or Nup53 under CUP1 promoter in log phase cells. (C) Western blot analysis of R2TP complex purified from log phase cells expressing Nup53-CΔ or Nup53. (D) Subcellular localizations of endogenously GFP-tagged Pih1 and Tah1 in crm1-T539C background strain in the absence or presence of LMB in stationary phase.

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