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. 2004 Jun;78(12):6543–6555. doi: 10.1128/JVI.78.12.6543-6555.2004

FIG. 3.

FIG. 3.

TRAIL is necessary but not sufficient to activate caspase-8 and to induce apoptosis in lyssavirus-infected neuroblastoma cells. Neuroblastoma cells were pretreated with anti-TRAIL antibodies (2.75 to 5.5 μg/ml; 1× to 2×), soluble TRAIL receptors (22 μg/ml), anti-FasL antibodies (11 to 22 μg/ml; 4× to 8×), or anti-TNF-α antibodies (22 μg/ml; 8×) for 1 h before virus infection (MOI, 1). They were maintained in media containing anti-ligands for 72 h. Results are means of data obtained in two independent experiments. Error bars, standard deviations.Significant effects are indicated by asterisks and pound signs (P < 0.05). (A and B) The percentage of apoptotic cells was evaluated by the TUNEL assay (A) and caspase-8 activity (B). (C) FACS analysis of cytosolic and cell surface TRAIL expression using a purified rat anti-mouse TRAIL monoclonal antibody or a purified rat monoclonal immunoglobulin isotype control followed by an FITC-conjugated anti-rat antibody. Data were collected (10,000 events) by using a Becton Dickinson FACScalibur and were analyzed with CellQuest software. NT, not treated; CONT, control.