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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Dec 5;391(1):984–989. doi: 10.1016/j.bbrc.2009.12.002

Fig. 2.

Fig. 2

Resveratrol induces endothelial KLF4 expression via a MEK5/MEF2-dependent, ERK5-independent pathway. (A) KLF4 mRNA expression in HUVEC infected with either control GFP or MEK5-DN adenovirus followed by incubation with 100 μM resveratrol or its vehicle. (B) KLF4 mRNA expression from HUVEC infected with either control GFP or MEK5-CA adenovirus. (C) Representative western blot showing the effect on ERK5 phosphorylation from HUVEC stimulated for 8 h with either vehicle, resveratrol (100 μM), or simvastatin (1.0 μM). (D) mRNA expression of KLF4 and ERK5 from HUVEC transfected with either ERK5 siRNA or control siRNA followed by incubation with 100 μM resveratrol or its vehicle (*p<0.01 vs. control + ctrl siRNA; #p<0.001 vs. respective ctrl siRNA group). Insert shows representative western blot demonstrating the silencing efficiency of ERK5. (E) Effect of MEF2ASA or GFP adenovirus on the induction of KLF4 mRNA expression by 100 μM resveratrol or its vehicle. All data are expressed as the mean +/- S.E.M. from three independent experiments (*p<0.01).