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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Sci Signal. 2014 Apr 15;7(321):ra36. doi: 10.1126/scisignal.2004762

Fig. 3. Changing the abundance of miR-3151 alters growth in part through TP53.

Fig. 3

(A) MV4-11 cells were stably infected with miR-3151, BAALC, scramble, or both miR-3151 and BAALC expression constructs (n = 3 biological replicates). Successfully infected cells (as determined by GFP positivity) were counted in duplicate for five consecutive days. (* indicates comparison with scramble; ** indicates comparison with miR-3151). (B) KG1a cells were stably infected with antagomiR-3151 or scramble expression constructs (n = 3 biological replicates). Cells were counted in duplicate for five consecutive days. (C) MV4-11 cells stably expressing miR-3151 or both miR-3151 and BAALC were transiently transfected with either vector containing TP53 or empty vector (EV). Starting at 24 hours after transfection, cells were counted in duplicate for five consecutive days. (* indicates comparison with miR-3151 + EV; ** indicates comparison with miR-3151/BAALC + EV). (D) TP53 was knocked down with siRNA in KG1a cells stably expressing antagomiR-3151 or scramble control constructs. Data in all panels are plotted as median cell counts on each day. All P values were determined by two-tailed Student’s t test. Error bars are omitted to improve clarity.