Skip to main content
. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Sci Signal. 2014 Apr 15;7(321):ra36. doi: 10.1126/scisignal.2004762

Fig. 5. Effect of miR-3151 on malignant melanoma cell lines.

Fig. 5

(A) Effect of overexpression of miR-3151 on TP53 transcript abundance (stable infection; mean ± SD, n = 3 biological replicates; P values were determined using two-tailed Student’s t test). (B) Western blot of TP53 abundance in A375 and MeWo cells stably expressing miR-3151 or the scramble control. The bar graphs depict the change in protein abundance as determined by densitometry (n = 3 blots; P values were determined by one-tailed Student’s t test). (C) CASP3/7 chemiluminescence assays of A375 and MeWo cells after stable infection with scramble control, miR-3151, or antagomiR-3151 (mean ± SD, n = 3 biological replicates; P values were determined using two-tailed Student’s t test). (D) Relative abundance of miR-3151 and BAALC in malignant melanoma patients (n = 19) according to their BRAF mutation status. Five patients had the BRAF mutation (mut) and 19 patients had wild-type BRAF (wt). Abundance of miR-3151 or BAALC transcripts in the wild-type patients was set to 1 (mean ± SEM; P values were determined using two-tailed Student’s t test).