FIG. 10.
Transduction of mouse hepatocytes following tail vein injection. A dose of 6.4 mg of mouse immunoglobulin was administered to an adult female nude mouse by means of tail vein injection to inhibit sequestration of the vector by Kupffer cells (35). After 6 h, HDA.Cre at 1.5 × 109 YTU was administered by means of tail vein injection. The mouse was sacrificed 19 days later, and 500-μm sections of liver embedded in agarose were prepared and examined for YFP fluorescence through a 15-μm z-axis stack under a fluorescein isothiocyanate channel by using a Bio-Rad confocal microscope. (A) Section from a control mouse injected with immunoglobulin only. (B) Section from a mouse injected with HDA.Cre at the same confocal settings as in panel A. (C) Same section as in panel B but at 38% the laser intensity used for panel B. Bar, 100 μm.