FIG. 2.

Generation of the EBV episome by the HDA-EBV hybrid system. D17 cells were infected with HDA.PAC+FR (MOI, 30) either alone (lane 2) or with HDA.Cre (MOI, 10) (lanes 3 to 5). Hirt DNA from infected cells was prepared at 24, 48, and 72 h p.i. as indicated, digested with BamHI, and analyzed by Southern blotting with the PAC fragment as a probe. This strategy allows distinction of the EBV episome (5.7-kb band) from the linear, unrecombined HDA.PAC+FR DNA (3.3-kb band). Lane 1 contains a marker of the plasmid DNA used to prepare HDA.PAC+FR cut with BamHI and PmeI.