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. 2004 Jun;78(12):6556–6566. doi: 10.1128/JVI.78.12.6556-6566.2004

FIG. 5.

FIG. 5.

(A) Generation of stable puromycin-resistant D17 transformants by the HDA-EBV hybrid system. D17 cells were coinfected with a constant amount of HDA.Cre (MOI, 10) and a range of MOIs for HDA.PAC+FR. Cells were selected in medium containing 1 μg of puromycin/ml starting at 7 days after the coinfection, and the appearance of colonies was monitored. The transformation efficiency was calculated as the number of colonies generated by infected cells in medium with puromycin divided by the number of colonies generated by mock-infected cells in medium without puromycin. (B) The cell plating efficiency as an estimate of cell viability was calculated by dividing the number of colonies generated by infected cells in medium without puromycin by the number of colonies formed by mock-infected cells in medium without puromycin. Error bars indicate standard deviations.