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. 2014 Sep 16;2:31. doi: 10.1186/2049-2618-2-31

Figure 2.

Figure 2

Oligonucleotides used in the study. The nucleotide positions for the primers and probe were numbered corresponding to the 1,542-bp 16S rRNA gene (rrnA) for Escherichia coli str. K-12 substr. MG1655 (NC_000913, nucleotide 4035531 to 4037072). ‡Degenerate nucleotides are shown in bold, Y for C or T, M for A or C. Sequences in blue, 454 sequencing system primers A or B. Sequences in red, 454 sequencing key for amplicon sequencing. The underlined sequence is the barcode, for which 454 Rapid Library Multiplex Identifiers (RLMIDs) was used. RL1 is shown as an example.