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. 2014 Aug 25;86(18):8974–8982. doi: 10.1021/ac502838t

Figure 2.

Figure 2

Workflow of the registration process. Spatially registering IMS data to anatomical data consists of 3 individual registration steps: (1) rigid registration of IMS data to experiment histology, (2) rigid registration of anatomical data to reference histology, and (3) nonrigid registration of experiment histology to reference histology.