Figure 3.

Overview of registration results. (A) Nonrigid registration of the experiment histology onto the reference histology from the atlas. (a) Despite the large initial deformation of the experiment histology, (b) the nonrigid transformation successfully compensates and (c) registers onto the (d) reference histology. (B) The nonrigid registration enables traversal from the coordinate system of one data source to the other: the acquired ion image can be projected onto the reference histology for direct comparison to the anatomical annotations. Inversely, the anatomical annotations, provided by the atlas, can be projected onto the experiment histology, to provide anatomical guidance within the sectioned tissue.