Table 2. Human PBMC proliferation in the presence of different antigens.
Prev - (%) | SI | |
---|---|---|
P181 | 92 | 9.8 |
P90 | 23 | 3.2 |
P77 | 54 | 3.1 |
P27 | 38 | 2.2 |
PBMC proliferation of adult donors living in Nigeria (N=13). Each antigen was put in culture in 200 μl at 10 μM (5 μM for P181) together with 2×105 PBMC at 37°C for 5 days. The cultures were then pulsed overnight with 3H-thymidine, and stimulation index (SI) was regarded significant when the radioactivity of stimulated over unstimulated cultures was ≥ 2.