| Cell Removing |
| Scratch Assay
|
scraping |
pipette tip, silicone wedge, (4-9) |
no |
scratch disrupts the substrate |
yes |
|
“scratch assay” |
488 |
| Electric Impedance
|
electric readout of cell adhesion and migration |
electric cell-substrate impedance sensing (ECIS), (11-12) |
no |
confluent cells alter the substrate |
no |
requires special plates with electrodes |
migration “cell-substrate impedance sensing” |
96 |
| Miscellaneous |
| Microfluidics
|
small fluidic channels and chambers |
cell trypsinization, cell patterning via capillary force, (13-14, 35-36) |
? |
varies with each device |
no |
requires microfluidic device and equipment |
microfluidic “wound healing” “cell migration” |
214 |
| Colony Migration
|
growth of small cell population into collective colony for migration analysis |
oil drop assay, collective migration on elastic substrate, (16,34) |
yes |
requires either exposing substrate to atmosphere or to oil drops while cells attach |
yes |
|
colony expansion “collective migration” |
17 |
| Cell Blocking |
| Stencils |
contact between stencil and substrate relies on auto-adhesion |
PDMS membrane, Culture-Inserts by Ibidi, (26-32) |
no |
substrate must be completely dry prior to attaching inserts |
yes |
requires uncoated or dry surface |
“collective cell migration” OR “collective migration” PDMS membrane OR stencil/Ibidi culture inserts migration |
121 |
| Stoppers |
wedging into specific size wells |
Cytoselect™ Wound Healing Inserts, Oris™ Cell migration assay, teflon stoppers, (22-25) |
? |
physical insertion of stoppers may disrupt the substrate |
no |
requires specific dimensions |
Oris cell migration/Cytoselect cell migration |
211 |
| Barricade Gels |
blocking cell adhesion with a dissolvable gel |
Radius™ Gel, Oris™ Pro (NA) |
no |
manufacturer determines substrate which must be dryable |
no |
sold in limited variety of plates |
“Radius gel” OR “Oris Pro” cell migration |
6 |
| MAts |
magnetic force maintains contact between stencil and substrate |
accepted and pending publication, (55) |
yes |
magnetic force attaches MAts without disrupting the substrate |
yes |
|
n/a developed in 2010 |
n/a |
