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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Nat Chem Biol. 2013 Dec 8;10(2):99–105. doi: 10.1038/nchembio.1411

Figure 1. A large repressor library is compiled using genome mining.

Figure 1

(a) A genetic NOT gate (symbol shown) can be built using a repressor (pink arrow) that binds to an operator (pink box) in an output promoter. (b) The pipeline for the discovery and characterization of orthogonal repressors is shown. The second panel depicts a portion of the CSI microarray used to determine the operator sequence. (c) The complete library of 73 synthesized repressors (plus TetR) are organized into a phylogenetic tree diagram, where carets indicate those repressors that appear in the final orthogonality matrix illustrated in Figure 3d. The tree was aligned based on respective repressor protein sequences and branch lengths correspond to relative divergence in amino acid sequence. The sequences and sources of each repressor are provided in Supplementary Data Set 1. The two IcaR orthologs originate from two distinct host organisms where (A) indicates Staphylococcus aureus and (E) indicates Staphylococcus epidermidis.