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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Nat Chem Biol. 2013 Dec 8;10(2):99–105. doi: 10.1038/nchembio.1411

Figure 2. The identification of operator sequences using an in vitro array assay.

Figure 2

(a) The hairpin sequences used to build the array correspond to a 28-mer inverted repeat sequence. N's indicate that all nucleotides are allowed at that position. The arrows above the hairpin mark each 14-mer half site and indicate the axis of symmetry for the palindrome. The variable region is surrounded by GC-clamps on both the 5’ and 3’ ends, and the hairpin contains a GGA loop to induce hairpin formation. The 3’ end is tethered to the array surface via a flexible linker. (b) Operators are shown for those repressors that yielded well-conserved sequence motifs.