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. 2004 Jun;78(12):6657–6665. doi: 10.1128/JVI.78.12.6657-6665.2004

FIG. 5.

FIG. 5.

(A) Peptide concentration dependence of KSHV Pr specific activity. The KSDD peptide inhibits KSHV Pr (filled circles) or KSHV Pr M197L (filled diamonds) activity by 50% at a ∼200- or 400-fold molar excess of peptide over KSHV Pr or KSHV Pr M197L, respectively. The inhibitory concentrations of the KSDD M17L peptide for KSHV Pr (open circles) and KSHV Pr M197L (open diamonds) were shifted lower than those of the KSDD peptide with the corresponding protease. In contrast, the α5 peptide did not inhibit KSHV Pr activity at concentrations up to 400-fold higher than KSHV Pr concentrations (open triangles). (B) Time dependence of KSDD peptide inhibition, assayed 0.08, 0.52, 0.92, 1.4, 1.9, 2.4, 3.1, 4.4, and 6.0 h after the mixing of 2 μM KSHV Pr with a 400-fold excess of the KSDD peptide, the highest concentration used in panel A.