FIG. 6.
(A) Comparison of the gel filtration profiles of 5 μM KSHV Pr (thick line) and KSHV Pr M197L (thin line). The dimerization affinity of KSHV Pr M197L is higher than that of KSHV Pr. (B) Stimulation of the specific activities of KSHV Pr (filled circles) and KSHV Pr M197L (open diamonds) upon dimerization (25). Assays were performed with 500 μM total substrate and 0.24 to 8 μM total enzyme. The best-fit Kd's for KSHV Pr and KSHV Pr M197L are 1.3 ± 0.24 and 0.40 ± 0.12 μM, respectively, as shown by the dashed lines.