Figure 3. Ploidy analysis in the C. elegans germ line (A and B) Regions of interests specifying nuclei in the pachytene (A) and proliferative (B) zone. The average nuclear intensity at the pachytene stage, which has known 4N content can be used normalize florescence values at the proliferative stage. (C) Normalized histograms. Normalized florescence values can be used to compare the distribution of DNA content within the proliferative region in WT and mutant worms. The rightward shift in the daf-2 distribution indicates an increased proportion of nuclei in late-S and G2.