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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Cell Signal. 2013 Apr 6;25(8):1665–1677. doi: 10.1016/j.cellsig.2013.03.020

Fig. 2.

Fig. 2

Cannabinoid-stimulated FAK phosphorylation at tyrosines 397 and 576/577 is mediated by CB1 receptors and Gi/o proteins in N18TG2 cells. Cells were pretreated with SR141716A (1 μM, 15 min) or pertussis toxin (100 ng/mL, 20 h) prior to treatment with 0.01 μM WIN55212-2 (WIN) for 1 or 2 min at 37°C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (A,C) pFAK tyrosine 576/577 levels and (B,D) pFAK tyrosine 397 levels (normalized to total FAK at each time point) from three separate experiments. WIN55212-2-stimulated values are expressed as 100%, and inhibitor-treated values are expressed as a percent of WIN55212-2-treated pFAK/FAK levels. * p < 0.001, # p < 0.01 indicates significantly different from WIN55212-2-stimulated at the same time point using Student's t-test.