Table 1.
The PCR primers used in multispacer typing and partially represented sequences deposited in GenBank of isolates tested
| Name of primer | Primer sequences 5'-3' (F/R) | Annealing temperature (°C) | Expected size (bp) | Actual size of the isolates tested | Sequences deposited in GenBank | Reference |
|---|---|---|---|---|---|---|
| dksA-xerC | TCCCATAGGTAATTTAGGTGTTTC/TACTACCGCATATCCAATTA | 54 | 416 | 225 | KJ461702 | [9, 10] |
| KJ461703 | ||||||
| KJ461704 | ||||||
| KJ461705 | ||||||
| KJ461706 | ||||||
| KJ461707 | ||||||
| KJ461708 | ||||||
| KJ461709 | ||||||
| spo0J-abcT1 | AAAGATTTGGAAGAATTAGACTTGAT/TTTGCTTAAACCAACCATTTCA | 50 | 320 | 254 | In process | [9] |
| RR0155-rpmB | TTTCTAGCAGCGGTTGTTTTATCC/TTAGCCCATGTTGACAGGTTTACT | 50 | 290 | 297 | In process | [11] |
| RR0345-tolC | AGAAGCTTCCGGATGTAATA/AGCAAATAAAAACCCTAATAAC | 50 | 238 | 238 | In process | [11] |
| cspA-ksgA | CATCACTGCTTCGCTTATTTT/ATTTCTTTTCTTCCTCTTCATCAA | 50 | 405 | 410 | KF923980 | [11] |
| KF923981 | ||||||
| KF923982 | ||||||
| KF923983 | ||||||
| KF923984 | ||||||
| KF923985 | ||||||
| KF923986 | ||||||
| KF923987 | ||||||
| KF923988 | ||||||
| KF923989 | ||||||
| KF923990 | ||||||
| KF923991 | ||||||
| KF923992 | ||||||
| KF923993 | ||||||
| KF923994 | ||||||
| KF923995 | ||||||
| KF923996 | ||||||
| KF923997 | ||||||
| KF923998 | ||||||
| KF923999 | ||||||
| KF924000 | ||||||
| KF924001 | ||||||
| RR1240-tlc5b | CGGGATAACGCCGAGTAATA/ATGCCGCTCTGAATTTGTTT | 50 | 357 | 366 | In process | [9] |
| RR1372-RR1373 | TCCCGCGCCAGTATCCA/CGGCGGCCAAAATGCTA | 50 | 349 | No product | In process | [11] |